Effect of Osmotic Pressure on the Activation and Storage of Channel Catfish Sperm
- 1 September 1996
- journal article
- Published by Oxford University Press (OUP) in Transactions of the American Fisheries Society
- Vol. 125 (5) , 798-802
- https://doi.org/10.1577/1548-8659(1996)125<0798:eoopot>2.3.co;2
Abstract
Artificial spawning of channel catfish Ictalurus punctatus relies on the removal of testis and suspension of sperm in an extender solution for storage and use in fertilization. Little is known about the relationships among osmotic pressure, sperm activation, motility, and storage. Our objectives were to (1) estimate motility of channel catfish sperm diluted in solutions ranging in osmotic pressure from 8 to 295 milliosmols (mosmol)/kg, (2) identify the osmotic pressure that induces threshold activation (10% motility) and the highest pressure that induces complete activation, (3) determine the role of ionic dilution in activation by use of ion-deficient solutions, and (4) evaluate the effect of osmotic pressure on the retention of motility during storage. Motility (percentage of actively swimming sperm) was estimated in diluted Hanks' balanced salt solution (HBSS) and sucrose solutions over a range of osmotic pressures. The HBSS osmotic pressures of threshold and complete activation were 218 ± 15 ...Keywords
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