Biological effects of bovine glia maturation factor on glial cells in culture
- 1 April 1981
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 6 (4) , 401-412
- https://doi.org/10.1007/bf00963855
Abstract
Optimal bioassay conditions for bovine glia maturation factor (GMF) were determined among glial cells from normal glioblasts to glioma cells. Rat glioblasts 4–8 days after subculture show the highest response to GMF with regard to morphological transformation and mitogenic activity. Bovine GMF enhances DNA synthesis of rat glioblasts at 12 hr after stimulation; maximum incorporation of [methyl-3H]thymidine was detected at 18 hr. GMF increases twofold the saturation density of rat glioblasts but does not alter that of C6 astrocytoma cells. The apparent inhibition of mitogenic activity of high doses of GMF is seen in both normal and malignant glial cells.This publication has 7 references indexed in Scilit:
- Glia maturation factor in bovine brain: Partial purification and physicochemical characterizationBrain Research, 1981
- Multiple molecular forms of Glia maturation factorBiochimica et Biophysica Acta (BBA) - Protein Structure, 1979
- Glia maturation factor increases cyclic GMP in glioblastsBrain Research, 1979
- Simplification of amino acid incorporation and other assays using filter paper techniquesAnalytical Biochemistry, 1977
- Glia Maturation Factor: Effect on Chemical Differentiation of Glioblasts in CultureScience, 1977
- Human epidermal growth factor and the proliferation of human fibroblastsJournal of Cellular Physiology, 1976
- Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growthNature, 1974