Thrombin and Phenotypic Modulation of the Endothelium
- 1 January 2004
- journal article
- review article
- Published by Wolters Kluwer Health in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 24 (1) , 41-53
- https://doi.org/10.1161/01.atv.0000099880.09014.7d
Abstract
Thrombin signaling in the endothelium is linked to multiple phenotypic changes, including alterations in permeability, vasomotor tone, and leukocyte trafficking. The thrombin signal is transduced, at least in part, at the level of gene transcription. In this review, we focus on the role of thrombin signaling and transcriptional networks in mediating downstream gene expression and endothelial phenotype. In addition, we report the results of DNA microarrays in control and thrombin-treated endothelial cells. We conclude that (1) thrombin induces the upregulation and downregulation of multiple genes in the endothelium, (2) thrombin-mediated gene expression involves a multitude of transcription factors, and (3) future breakthroughs in the field will depend on a better understanding of the spatial and temporal dynamics of these transcriptional networks.Keywords
This publication has 170 references indexed in Scilit:
- Thrombin and Protease-Activated Receptor-1 Agonists Promote Lipopolysaccharide-Induced Hepatocellular Injury in Perfused LiversThe Journal of Pharmacology and Experimental Therapeutics, 2003
- Activated protein C blocks p53-mediated apoptosis in ischemic human brain endothelium and is neuroprotectiveNature Medicine, 2003
- A potential role for nuclear factor of activated T-cells in receptor tyrosine kinase and G-protein-coupled receptor agonist-induced cell proliferationBiochemical Journal, 2002
- Thrombin-stimulated Pyk2 phosphorylation in human endothelium is dependent on intracellular calcium and independent of protein kinase C and Src kinasesBiochemical and Biophysical Research Communications, 2002
- Induction of KDR expression in bovine arterial endothelial cells by thrombin: Involvement of nitric oxideJournal of Cellular Physiology, 2002
- Endothelial exposure to hypoxia induces Egr‐1 expression involving PKCα‐mediated Ras/Raf‐1/ERK1/2 pathwayJournal of Cellular Physiology, 2001
- Identification of Thrombin Receptors in Rat Brain Capillary Endothelial CellsJournal of Cerebral Blood Flow & Metabolism, 2000
- Reverse transcriptase—polymerase chain reaction (RT—PCR): a sensitive method to examine basic fibroblast growth factor-induced expression of the early growth response gene-1 (egr-1) in human umbilical arterial endothelial cellsMolecular and Cellular Probes, 1995
- Protein kinase C phosphorylates caldesmon77 and vimentin and enhances albumin permeability across cultured bovine pulmonary artery endothelial cell monolayersJournal of Cellular Physiology, 1992
- Difference between Endothelium-Dependent Relaxation in Arterial and in Venous Coronary Bypass GraftsNew England Journal of Medicine, 1988