Initiation of plasmid pC194 replication and its control in Bacillus subtilis
- 1 December 1987
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 210 (3) , 476-484
- https://doi.org/10.1007/bf00327200
Abstract
By deletion analysis we have defined a 1.1 kb segment required for driving autonomous replication of the plasmid pC194. The minimal replicon specifies a positive, RepH, and a negative, Inc8A, trans-acting product and their target sites. The RepH product has a M4 of 34.1 kDa, could be overproduced, and binds specifically to the pC194 origin region. By trans complementation studies we have shown that pC194 replication is indirectly controlled at the level of RepH synthesis by a negative product, IncA, that is transcribed within the repH transcription unit in the opposite direction (“antisense RNA”). The antisense RNA regulates the RepH synthesis by a mechanism that seems to involve RNA/RNA interaction in a manner that interferes with translation. In addition, an autoregulatory control might be operative.Keywords
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