Cycling S-Phase Cells in Animal and Spontaneous Tumours: I. Comparison of the BrdUrd and 3H-thymidine techniques and flow cytometry for the estimation of S-phase frequency
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Acta Radiologica: Oncology
- Vol. 24 (6) , 545-548
- https://doi.org/10.3109/02841868509134429
Abstract
Evaluation of the proliferative activities of cell populations has mainly been restricted to the use of autoradio-graphy and flow cytometric measurements. The introduction of a new BrdUrd specific antibody makes it possible to determine exactly the DNA synthesizing cells. The BrdUrd technique is safe with respect to handling and the results are obtained within five hours. The suitability of the BrdUrd labelling procedure has been studied in different cell lines and compared with 3H-thymidine autoradio-graphy and flow cytometry.This publication has 1 reference indexed in Scilit: