FLOW MICRO-CALORIMETRIC STUDY OF THE EFFECTS OF PH, METAL-IONS AND STEROLS ON THE INTERACTION OF POLYENE ANTIBIOTICS WITH SACCHAROMYCES-CEREVISIAE NCYC239
- 1 January 1980
- journal article
- research article
- Vol. 28 (112) , 107-121
Abstract
The effects of pH, metal ions (Ca and Mg) and sterols (cholesterol and ergosterol), on the interaction of polyene antibiotics with respiring S. cerevisiae (NCYC 239) cells were studied by flow microcalorimetry. The bioactivity of nystatin decreases as the pH of the medium increases toward neutral. Ca and Mg ions attenuate the biological effects of nystatin, amphotericin B and candicidin. The activities of filipin and pimaracin (natamycin) are unaffected by the presence of these ions. Ergosterol and cholesterol show similar effects. Some possible explanations for these observations are suggested.This publication has 7 references indexed in Scilit:
- Combined microassay and determination of bioactivity of N-acetylnystatinTalanta, 1980
- Factors Influencing the Susceptibility of Candida albicans to the Polyenoic Antibiotics Nystatin and Amphotericin BJournal of General Microbiology, 1978
- Bioassay of antifungal antibiotics by flow microcalorimetryAnalytical Chemistry, 1977
- Bioassay of nystatin bulk material by flow microcalorimetryAnalytical Chemistry, 1976
- Application of Flow Microcalorimetry to Analytical Problems: the Preparation, Storage and Assay of Frozen Inocula of Saccharomyces cerevisiaeJournal of Applied Bacteriology, 1976
- Differential Effects of Monovalent and Divalent Ions upon the Mode of Action of the Polyene Antibiotic CandicidinJournal of Applied Bacteriology, 1976
- Effect of the lipid composition of Mycoplasma mycoides subspecies Capri and phosphatidylcholine vesicles upon the action of polyene antibioticsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976