Reduction of trapping of aggregated human IgG in germinal centers by mitogens

Publisher Website
Google Scholar
Abstract
The effect of nonspecific mitogens on the trapping of 125I‐labeled aggregated human IgG (125I‐AHGG) in germinal centers (GC) of mouse spleens has been investigated by both radioactivity uptake and immunofluorescence. Phytohemagglutinin and concanavalin A (Con A) significantly decreased trapping. Lipopolysaccharide produced less inhibition, and pokeweed mitogen had no significant effect. The maximum inhibition occurred with 250–500 μg Con A. This had no effect on 125I‐AHGG uptake in liver, kidney and blood. No differences were found between i.p. and i.v. routes of Con A injection. The effect of mitogens on the 125I‐AHGG trapping in GC is due more likely to modification of the migratory properties of lymphocytes brought about by surface binding, than to their mitogenic properties, since Con A decreased 125I‐AHGG localization in thymectomized, x‐irradiated and bone marrow‐ reconstituted animals.