Artificial Transformation of O Antigens in Salmonella E Group. II. Antigen-Transforming Factor in Bacilli of Subgroup E2

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Abstract
In autolysates of S. newington, S. selandia and S. new-Brunswick of subgroup E2 of Salmonella E group we found the filtrable factor which transformed III, X antigen contained in S. london, S. give and S. anatum of subgroup E1 into III, XV antigen contained in bacilli of subgroup E2. And this antigen-transforming factor was difficult to separate from the bacteriophage liberated by bacilli of subgroup E2 and acting to bacilli of subgroup E1. Further, there was always agreement between the intensities of both actions, and the phage antibody caused the disappearance of the ability of antigen-transforming factor. From these facts we consider the antigen-transforming factor to be the phage itself. Namely, the phage-resistant bacilli which derived from bacilli of subgroup E1 by the action of the phage liberated by lysogenic strains of subgroup E2 have the O antigen III, XV transformed by phage. At the same time they become lysogenic strains, produce the phage, and come to have an activity to transform O antigen by acting upon bacilli of subgroup E1. Therefore, when we made phage antibody act upon bacilli of subgroup E2, then by the transformation of antigen, we could easily produce bacilli which contained III, X antigen of subgroup E1. Further, we made anti-I, XIX serum act upon S. senftenberg (I, III, XIX) of subgroup E3 and obtained bacilli which came to have III, XV antigen by antigenic transformation. These bacilli came to have phage against bacilli of subgroup E1 and exhibited antigen-transforming activity.

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