Expression of mRNAs of multidrug resistance proteins (Mrps) in cultured rat astrocytes, oligodendrocytes, microglial cells and neurones
Open Access
- 24 July 2002
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 82 (3) , 716-719
- https://doi.org/10.1046/j.1471-4159.2002.01082.x
Abstract
Multidrug resistance proteins (Mrps) are ATP‐driven export pumps that mediate the export of organic anions from cells. So far only little information is available on expression and physiological functions of Mrps in brain. The expression of mRNAs of six Mrp paralogs in rat brain, as well as in rat cultures enriched for neurones, astrocytes, oligodendrocytes and microglial cells, was studied by qualitative and semiquantitative RT‐PCR analysis. In adult rat brain as well as in neural cell cultures the mRNAs coding for Mrp1, Mrp3, Mrp4 and Mrp5 were detected. Semiquantitative analysis revealed that the mRNAs coding for Mrp1 and Mrp5 were more abundant in the four cell culture types than mRNAs of the other Mrps. mRNAs coding for Mrp3 and Mrp4 were found at significant levels in cultured astrocytes and microglial cells, whereas cultures of neurones and oligodendrocytes contained only marginal quantities of these mRNAs. Putative physiological functions of Mrps in brain cells are discussed.Keywords
This publication has 32 references indexed in Scilit:
- Oligodendroglial cells in culture effectively dispose of exogenous hydrogen peroxide: comparison with cultured neurones, astroglial and microglial cellsJournal of Neurochemistry, 2002
- Toxicological relevance of the multidrug resistance protein 1, MRP1 (ABCC1) and related transportersToxicology, 2001
- Transport of Cyclic Nucleotides and Estradiol 17-β-d-Glucuronide by Multidrug Resistance Protein 4Journal of Biological Chemistry, 2001
- Microglial Cells in Culture Express a Prominent Glutathione System for the Defense against Reactive Oxygen SpeciesDevelopmental Neuroscience, 2000
- The Multidrug Resistance Protein 5 Functions as an ATP-dependent Export Pump for Cyclic NucleotidesJournal of Biological Chemistry, 2000
- Functional expression of p-glycoprotein and multidrug resistance-associated protein (mrp1) in primary cultures of rat astrocytesJournal of Neuroscience Research, 2000
- Glutathione levels in primary glial cultures: Monochlorobimane provides evidence of cell type-specific distributionGlia, 1999
- Expression of Multidrug Resistance-Associated Protein (MRP) in Brain Microvessel Endothelial CellsBiochemical and Biophysical Research Communications, 1998
- Overexpression of a Transporter Gene in a Multidrug-Resistant Human Lung Cancer Cell LineScience, 1992
- [27] Primary glial cultures as a model for studying hormone actionPublished by Elsevier ,1985