Adaptive aberration correction in a two‐photon microscope
- 1 November 2000
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 200 (2) , 105-108
- https://doi.org/10.1046/j.1365-2818.2000.00770.x
Abstract
We demonstrate aberration correction in two-photon microscopy. Specimen-induced aberrations were measured with a modal wavefront sensor, implemented using a ferro-electric liquid crystal spatial light modulator (FLCSLM). Wavefront correction was performed using the same FLCSLM. Axial scanned (xz) images of fluorescently labelled polystyrene beads using an oil immersion lens show restored sectioning ability at a depth of 28 µm in an aqueous specimen.Keywords
This publication has 14 references indexed in Scilit:
- New modal wave-front sensor: a theoretical analysisJournal of the Optical Society of America A, 2000
- Imaging properties in two-photon excitation microscopy and effects of refractive-index mismatch in thick specimensApplied Optics, 1999
- Dynamic wave-front generation for the characterization and testing of optical systemsOptics Letters, 1998
- Aberration correction for confocal imaging in refractive‐index‐mismatched mediaJournal of Microscopy, 1998
- Simple binary optical elements for aberration correction in confocal microscopyOptics Letters, 1995
- Fundamental Limits in Confocal MicroscopyPublished by Springer Nature ,1995
- Refractive-index-induced aberrations in two-photon confocal fluorescence microscopyJournal of Microscopy, 1994
- Aberrations in confocal fluorescence microscopy induced by mismatches in refractive indexJournal of Microscopy, 1993
- Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopyJournal of the Optical Society of America A, 1991
- Two-Photon Laser Scanning Fluorescence MicroscopyScience, 1990