Effect of Chlorides and Heat on Extraction and Activity of Sheep Pituitary Gonadotrophin.

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Abstract
Concentrated fractions of dry sheep pituitary tissue obtained from extracts made at 70[degree]C with water, saturated solns. of NaCl and KC1, and with 0.88 [image] sucrose contained the major part of the gonadotrophic activity. Fractions obtained from extracts made with saturated solns. of BaCl2, CaCl2, and MgCl2, and 6.6 [image] urea at 70[degree]C were much less active. Dialyzed soluble fractions obtained from saturated NaCl extracts made at 80[degree]C contained little activity and those from extracts made at 90[degree]C were essentially inactive. Extraction at 70[degree]C with the satd. solns. and fractionation of the dialyzed extracts did not separate FSH from LH. The activity of the gonadotrophin was augmented when administered in solns. which were 0.5 saturated with NaCl and KC1.