Monohydroperoxides of linoleic acid in endoplasmic lipids of rats exposed to tetrachloromethane

Abstract
An accurate method for the quantitative determination of hydroperoxy and hydroxy fatty acids in liver microsomes is presented which involves the use of18O-labeled internal standards. The method is employed for the determination of hydroperoxides in rat liver microsomes after aerobic incubation with Fe2+/ADP and in microsomes from animals exposed to 75 mg tetrachloromethane/kg body weight. The amounts found after artificial microsomal “lipid peroxidation” are almost two orders of magnitude larger than those in microsomes from tetrachloromethaneexposed animals.

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