The effect of hind-limb ischaemia on the products and intermediates of glycolysis in rat tissues

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Abstract
The early effects of bilateral hind-limb ischaemia on the concentrations of phosphorylated glycolytic intermediates, pyruvate, lactate and [alpha]-oxoglutarate, in muscle and liver have been investigated in intact, intact cold-acclimatized, adrenal-medullectomized and adrenal-ectomized rats at air temperatures between 18[degree] and 22[degree] and also in intact rats at an air temperature of 30[degree]. The changes in the phosphorylated glycolytic intermediates in the injured muscle during ischaemia were the same as those reported for anoxic muscle in vitro. In the uninjured fore-limb muscle the fructose 1,6-diphosphate and pyruvate concentrations rose after hind-limb ischaemia. These changes were attributed to increased glycogenolysis and to decreased utilization of fructose, 1,6-diphosphate and possibly also of pyruvate. The most constant change in the liver after limb ischaemia was an increase in the pyruvate concentration and also in that of fructose 1,6-diphosphate when conditions were such that the liver temperature fell. These changes are attributed to a decrease in the utilization of fructose 1,6-diphosphate and in the oxidation of pyruvate.