Epidermal Growth Factor Induces the Expression of Its Receptor Gene in Human Gastric Carcinoma Cell Line TMK‐1

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Abstract
To ascertain the possible autocrine pathway in the growth promotion of gastric carcinomas, a study was made on the effects of exogenous human epidermal growth factor (hEGF) on the expression of mRNA for EGF, transforming growth factor-.alpha. (TGF-.alpha.), EGF receptor, FOS and MYC genes by TMK-1 cells. Exogenous hEGF increased FOS and MYC mRNA levels 30 min and 1 h after the treatment, respectively. TMK-1 cells accumulated the mRNA for EGF receptor about 7- to 8-fold by 3 h after treatment. Expressions of mRNA for EGF and TGF-.alpha. genes were detected, but the amounts of the mRNA of these genes in TMK-1 cells were not altered after the treatment.