17-KETOSTEROIDS AND PSEUDO KETOSTEROIDS IN THE URINE OF CATTLE
- 1 September 1957
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 26 (1) , 75-90
- https://doi.org/10.1530/acta.0.0260075
Abstract
For the colorimetric estimation of the urinary excretion of 17-ketosteroids (17-KS) in cattle, the Zimmerman method was used. Not all "Zimmermann positive substances" were 17-KS as 90% of the total ketosteroid value was due to non-steroidal substances. The total concentration of 17-KS was 0.3 mg/1 of urine. According to paper chromatography, the 17-KS identified were dehydroepiandrosterone, etiocholanolone and 11-ketoetiocholanolone. There was an increase in 17-KS excretion after testosterone or cortiocotropin was injected. The main compounds from the neutral lipid fraction of cattle urine were ionone derivatives, presumably metabolities of carotenoids found in cattle fodder.Keywords
This publication has 15 references indexed in Scilit:
- Untersuchungen über Organextrakte und Harn. 19. Mitteilung. Über die Konstitution der Oxy‐ketone E und G aus dem Harn von trächtigen StutenHelvetica Chimica Acta, 1950
- Untersuchungen über Organextrakte und Harn. 16. Mitteilung. Über die Konstitution des ungesättigten Ketons C13H18O aus dem Harn trächtiger StutenHelvetica Chimica Acta, 1949
- A NEW METHOD FOR THE SEPARATION OF ANDROGENS FROM ESTROGENS AND FOR THE PARTITION OF ESTRIOL FROM THE ESTRONE-ESTRADIOL FRACTION - WITH SPECIAL REFERENCE TO THE IDENTIFICATION AND QUANTITATIVE MICRODETERMINATION OF ESTROGENS BY ULTRAVIOLET ABSORPTION SPECTROPHOTOMETRY1948
- STUDIES IN STEROID METABOLISM .1. METHODS FOR THE ISOLATION AND QUANTITATIVE ESTIMATION OF NEUTRAL STEROIDS PRESENT IN HUMAN URINE1948
- Untersuchungen über Organextrakte. 13. Mitteilung. Über die Isolierung von Jonon-Derivaten aus dem Harn trächtiger StutenHelvetica Chimica Acta, 1948
- Untersuchungen über Organextrakte. (11. Mitteilung). Über die Konstitution des Alkohols C13H20O aus dem Harn trächtiger StutenHelvetica Chimica Acta, 1947
- CLINICAL METHOD FOR THE CHROMATOGRAPHIC-COLORIMETRIC DETERMINATION OF URINARY 17-KETOSTEROIDS. I.Journal of Clinical Endocrinology & Metabolism, 1946
- NEW COLOR REACTION FOR CERTAIN URINARY 17-KETOSTEROIDS1Endocrinology, 1943
- Colorimetric determination of substances containing the grouping —CH2.CO— in urine extracts as an indication of androgen contentBiochemical Journal, 1938
- CLINICAL STUDIES OF THE BLOOD VOLUME. IV. ADAPTATION OF THE METHOD TO THE PHOTOELECTRIC MICROCOLORIMETERJournal of Clinical Investigation, 1938