NON‐ANTIBODY COMPONENTS IN HUMAN MILK INHIBIT ESCHERICHIA COLI HEAT LABILE ENTEROTOXIN MEASURED BY AN ENZYME‐LINKED IMMUNOSORBENT ASSAY

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Abstract
Milk from 11 Norwegian women was fractionated by (NH4)2 SO4 precipitation and column chromatography. The milk samples inhibited the binding of heat labile E. coli enterotoxin to antibodies coated on microtiterplates in an enzyme-linked immunosorbent assay. Inhibiting activity was not detected when the toxin was measured in an adrenal cell assay. The inhibiting activity was of a non-Ig nature with an apparent MW of > 400,000 in gel filtration experiments.