Crystallisation of the Bacillus subtilis sporulation inhibitor SinR, complexed with its antagonist, Sinl
Open Access
- 2 January 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 378 (1) , 98-100
- https://doi.org/10.1016/0014-5793(95)01432-2
Abstract
The transcription factor SinR, a pleiotropic regulator of late growth processes in Bacillus subtilis, has been crystallised as a complex with its antagonist Sinl, in a form suitable for structural analysis. The Sinl : SinR crystals diffract X‐rays generated from a rotating copper anode source to 2.3 Å spacing and a complete native dataset has been collected to this resolution limit. The space group of the crystals is P3121 (or its enantiomorph P3221) with cell dimensions . Assuming that there is a single Sinl: SinR heterodimer in the asymmetric unit, the crystals have a V m of 2.53 Å3 · Da−1.Keywords
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