Retardation of Senescence in Red Clover Leaf Discs by a New Antiozonant, N-[2-(2-Oxo-1-imidazolidinyl)ethyl]-N′-phenylurea

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Abstract
Dark-induced senescence in leaf discs from O3-sensitive red clover trifoliates (Trifolium pratense L. cv. `Pennscott') was markedly retarded by treatment with N-[2-(2-oxo-1-imidazolidinyl)ethyl-N′-phenylurea (EDU). EDU also protects against acute and chronic foliar O3 injury when sprayed on intact leaves or supplied to the plants through soil application. Senescence retardation was measured by time-dependent analyses of chlorophyll, protein, and RNA in discs floated on aqueous EDU solutions ranging from 0 to 500 micrograms per milliliter EDU. Chlorophyll degradation, total protein, and nucleic acids were followed over 10-day test periods.