A transgenic mouse model for monitoring endoplasmic reticulum stress
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- 14 December 2003
- journal article
- Published by Springer Nature in Nature Medicine
- Vol. 10 (1) , 98-102
- https://doi.org/10.1038/nm970
Abstract
Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. Although the connection between ER stress and some disease-related proteins has been studied using animal models of these diseases, no in vivo data concerning ER stress are available. Here we report a new method for monitoring ER stress in vivo, based on XBP-1 mRNA splicing by inositol requiring-1 (IRE-1) during ER stress. The stress indicator was constructed by fusing XBP-1 and venus, a variant of green fluorescent protein. During stress, the spliced indicator mRNA is translated into an XBP-1-venus fusion protein, which can be detected by its fluorescence. We used transgenic animals expressing the ER stress indicator to show that it can be used to monitor physiological and pathological ER stress in vivo.Keywords
This publication has 25 references indexed in Scilit:
- Proteasome inhibitors disrupt the unfolded protein response in myeloma cellsProceedings of the National Academy of Sciences, 2003
- ASK1 is essential for endoplasmic reticulum stress-induced neuronal cell death triggered by expanded polyglutamine repeatsGenes & Development, 2002
- IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNANature, 2002
- A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applicationsNature Biotechnology, 2002
- Complementary signaling pathways regulate the unfolded protein response and are required for C. elegans development.Published by Elsevier ,2001
- XBP1 mRNA Is Induced by ATF6 and Spliced by IRE1 in Response to ER Stress to Produce a Highly Active Transcription FactorCell, 2001
- Coupling Endoplasmic Reticulum Stress to the Cell Death ProgramJournal of Biological Chemistry, 2001
- Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-βNature, 2000
- Homocysteine and Cardiovascular DiseaseAnnual Review of Medicine, 1998
- Homocyst(e)ine: an important risk factor for atherosclerotic vascular diseaseCurrent Opinion in Lipidology, 1997