The Genome-Linked Protein of Picornaviruses
- 1 January 1980
- journal article
- research article
- Published by S. Karger AG in Intervirology
- Vol. 13 (3) , 192-199
- https://doi.org/10.1159/000149125
Abstract
Digestion of poliovirus type 2[32P]-RNA with enzymes and analysis of the products by column chromatography and paper electrophoresis provides evidence that the RNA is covalently linked to a small, basic protein, VPg, via a 5''-terminal pU residue. Digestion of the RNA with proteinase K followed by labeling of the peptidyl-RNA with the Bolton and Hunter reagent [iodinated 3-(4-hydroxyphenyl)propionic acid N-hydroxysuccinimide ester] yields 5''-labeled material suitable for rapid sequencing. The 5''-terminal sequence of poliovirion type 2 RNA was determined to be VPg-pUUAAAACAG... which is identical to the sequence at the 5''-terminus of the poliovirus type 1 genome.This publication has 13 references indexed in Scilit:
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