SEPARATION AND ESTIMATION OF MB ISO-ENZYME OF CREATINE-PHOSPHOKINASE IN SERUM - RESULTS IN 152 CASES

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  • 1 January 1975
    • journal article
    • research article
    • Vol. 33  (5) , 385-390
Abstract
MB iso-emzyme of creatine phosphokinase (CPK) was estimated after chromatography. Mercer''s technique, was simple and rapid. It required 1 ml of serum. The study carried out in 152 subjects showed that CPK was increased (N < 90 mU/ml). In the group of 104 patients with myocardial infarction (average CPK levels = 829 mU/ml .+-. 516), the MB iso-enzyme was in the serum in signifcant levels (average level of the MB fraction = 10.9% .+-. 5.4). On the other hand, in the control group of 44 patients with various diseases, (CPK = 672 mU/ml .+-. 531) the MB iso-enzyme remained low (0.35% .+-. 0.44). In 2 subjects with myocardial infarction, no MB fraction was found. In 2 patients who died, 1 from necrotic, prostatic adenocarcinoma, the other from necrotic epidermoid lung cancer, the MB iso-enzyme was significantly increased. The test proposed here, which is applicable in a routine laboratory, seems the most specific of all laboratory examinations available to the clinician for the diagnosis of myocardial infarction.

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