Cytogenetic Analyses by FluorescenceIn SituHybridization (FISH) in Hospital Workers Occupationally Exposed to Low Levels of Ionizing Radiation

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Abstract
Cigarrán, S., Barquinero, J. F., Barrios, L., Ribas, M., Egozcue, J. and Caballín, M. R. Cytogenetic Analyses by Fluorescence In Situ Hybridization (FISH) in Hospital Workers Occupationally Exposed to Low Levels of Ionizing Radiation. An occupationally exposed population has been studied to evaluate the suitability of FISH painting techniques to detect chronic exposures to very low doses of ionizing radiation by the analysis of translocations. Whole-chromosome painting probes for chromosomes 1, 4 and 11 in combination with a pancentromeric probe have been employed. For comparison, a matched control population has also been studied. The mean genomic frequencies per 100 cells of total translocations in the control and exposed populations were 0.90 ± 0.12 and 1.04 ± 0.11, respectively. In the occupationally exposed population, no correlation between the frequencies of translocations and the doses received was found. When the two populations were compared, no significant differences were observed for the frequencies of the different chromosomal abnormalities examined. The absence of differences between control and exposed populations could be attributed to the very low-dose exposures recorded in the occupationally exposed population and to the wide range of individual frequencies of translocations observed.

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