Crystallisation and preliminary X-ray analysis of the receptor-binding domain of human and bovineα2-macroglobulin
- 18 September 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 372 (1) , 93-95
- https://doi.org/10.1016/0014-5793(95)00960-h
Abstract
The receptor-binding domains (RBDs) of human and bovine α 2 -macroglobulin ( α 2 M) have been isolated after limited proteolysis of methylamine-treated α 2 M with papain. Single crystals of the RBDs have been grown by vapour diffusion. Crystals of human RBD are very thin plates unsuited for data collection. However, crystals of RBD from bovine α 2 M give diffraction patterns suitable for X-ray analysis, and a complete dataset with a maximum resolution of 2.3 Å has been collected with synchrotron radiation at cryogenic temperature. The crystals belong to spacegroup P3 1 21 or P3 2 21 with cell parameters a = b = 106.8 A ̊ , c = 72.2 A ̊ .Keywords
This publication has 15 references indexed in Scilit:
- The α-Macroglobulin Bait Region: Sequence diversity and localization of cleavage sites for proteinases in five mammalian α-macroglobulinsPublished by Elsevier ,2021
- Localization of .epsilon.-lysyl-.gamma.-glutamyl cross-links in .alpha.2-macroglobulin-plasmin complexBiochemistry, 1993
- Evidence that the newly cloned low‐density‐lipoprotein receptor related protein (LRP) is the α2‐macroglobulin receptorFEBS Letters, 1990
- Mounting of crystals for macromolecular crystallography in a free-standing thin filmJournal of Applied Crystallography, 1990
- The structure of α2-macroglobulin-methylamine after papain digestion as determined by electron microscopyBiochemical Journal, 1990
- Determination of amino acid compositions and NH2-terminal sequences of peptides electroblotted onto PVDF membranes from Tricine-Sodium dodecyl sulfate-polyacrylamide gel electrophoresis: Application to peptide mapping of human complement component C3Analytical Biochemistry, 1989
- α-Macroglobulins: structure, shape, and mechanism of proteinase complex formationJournal of Biological Chemistry, 1989
- Domain structure of human α2‐macroglobulinFEBS Letters, 1986
- Residual α2-macroglobulin in fetal calf serum and properties of its complex with thrombinBiochemical and Biophysical Research Communications, 1982
- A thiol‐ester in α2‐macroglobulin cleaved during proteinase complex formationFEBS Letters, 1980