COMPARISON OF NORMAL AND CHRONIC LYMPHOCYTIC LEUKEMIA LYMPHOCYTE SURFACE IG DETERMINANTS USING PEROXIDASE-LABELED ANTIBODIES .2. QUANTIFICATION OF LIGHT CHAIN DETERMINANTS IN ATYPICAL LYMPHOCYTIC LEUKEMIA

  • 1 January 1976
    • journal article
    • research article
    • Vol. 48  (4) , 559-566
Abstract
Five cases of atypical lymphocytic leukemia were investigated with regard to their membrane-associated L chains. Detection and quantitation of antigenic determinants were performed by peroxidase-labeled antibodies according to Avrameas et al. The cases studied had clinical and cytologic features in common: an active clinical course, marked splenomegaly, severe anemia and thrombocytopenia, little or no lymph node enlargement and high white blood cell counts with small mature lymphocytes and poorly differentiated lymphoid cells. Blood lymphocytes of all patients carried a single type of L chain, and 90%-100% of the cells were stained. The average number of antigenic sites per cell was 72,500 (range 40,000-97,500). These results differed from those previously found in typical CLL [chronic lymphocytic leukemia] (mean value 9000) and approached the values of normal peripheral blood lymphocytes (90,000). The criteria investigated in this study could be of value for the diagnosis and prognosis of some atypical forms of lymphocytic leukemia.

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