THE DETERMINATION OF FREE 17,21-DIHYDROXY-20-KETOSTEROIDS IN SMALL AMOUNTS (4 ml.) OF PERIPHERAL BLOOD

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Abstract
A modification of the method of Silber and Busch (1956) for the determination of free 12, 21-dihydroxy-20-ketosteroids in plasma is described. The method requires only 4 ml of peripheral blood, i.e. 1/5 of the original amount used. Construction of a special plunger for addition of the 0.07-0.08 ml samples to the cuvettes is described in detail. For accurate results, the use of standards made up in washed plasma was found to be necessary. Because less 17-OH-CS can be removed from plasma than from water, aqueous standards give results which are too low. The main advantage of the micro modification described is that it enables repeated determinations at short intervals in the same subject.

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