Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control

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Abstract
Bacteriophage vectors derived from λplac5 have been constructed. Their genomes have one EcoRI restriction site which is located at the very beginning of the lac Z gene. The major part of this gene was deleted by an in vivo intramolecular recombination. These vectors allow the fusion of a gene or an operon with the beginning of the lac Z gene, placing them under the control of the lac promoter, which carries the UV5 mutation. Some of these vectors (λY) also include the lac Y gene and it too is under the control of the lac promoter. The λYEQS, which carries the Qam73 and Sam7 mutations, as well as safety mutations, has been certified as a B2 (EK2) vector by the French control commission “recombinaison génétique in vitro”.