Quantitative Studies of Japanese B Encephalitis Virus in Hamster Kidney Cell Cultures.

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Abstract
Japanese-B encephalitis virus was studied in hamster kidney cell (HKC) cultures by the plaque method. The effect of some variables on plating efficiency were: Maximum adsorption was obtained by 120 min. incubation at 35[degree]C. Optimal conditions for initiating plaques were obtained by seeding virus in a small volume of lactalbumin medium containing bovine albumin. Cations were required for maximum virus adsorption but plaques were produced without added CA++ and Mg++. Washing cultures with phosphate-buffered saline (pH 7.0) before and after exposure to virus were not required, and plaques developed from virus adsorbed on cells before agar was applied. The titers obtained by plaque assay were similar to those of tube cultures and varied with mouse titer and passage level in mice or in HKC. HCK proved slightly more sensitive for plaque assay then chick embryo cells with HKC adapted virus.