THE SEPARATION OF ORCEIN INTO FOUR FRACTIONS BY CHROMATOGRAPHY AND THE STAINING QUALITIES OF EACH FRACTION
- 1 January 1954
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 2 (1) , 9-20
- https://doi.org/10.1177/2.1.9
Abstract
The absorption spectra, paper chromatography, column chromatography, paper electrophoresis, and staining characteristics of synthetic and natural orcein were studied. The following conclusions were reached: (1) Orcein is a mixture of closely related amphoteric dyes which may be separated into 4 principal colored fractions; (2) The same fractions are present in orcein from natural as well as from synthetic sources; (3) Orcein staining of tissue is relatively insensitive to pH and therefore probably reacts through non-ionic bonds. In addition, Fraction II reacts as a modified basic dye; (4) Whole orcein or its fractions are excellent general tissue stains as well as elastic tissue and chromosome stains. Fraction IV has poor staining qualities.Keywords
This publication has 2 references indexed in Scilit:
- Characterization of the reaction between orcein and the elastic fibers of the ligamentum nuchae of the horseThe Anatomical Record, 1952
- A Revised Method for the “Quad” StainStain Technology, 1945