Lipid-Linked Desaturation of Palmitic Acid in Monogalactosyl Diacylglycerol in the Blue-Green Alga (Cyanobacterium) Anabaena variabilis Studied in Vivo
- 1 July 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 27 (5) , 819-835
- https://doi.org/10.1093/oxfordjournals.pcp.a077168
Abstract
The mechanism of desaturation of palmitic acid in monogalactosyl diacylglycerol in Anabaena variabilis was studied by labeling in vivo with 13C and mass spectrometry. When the cells were fed with [13C]Na2CO3 for 2.5 h, 19% of the palmitic, but virtually none of the palmitoleic, acid at the C-2 position of the lipid was enriched with 13C. During subsequent incubation for 7.5 h, the [13C]palmitic acid was desaturated to [13C]palmitoleic acid. Mass spectrometric analysis of the 2-acylglycerol moiety of the lipid indicated that [13C]palmitoyl-[13C]glycerol and [12C]palmitoyl-[12C]glycerol were converted to [13C]palmitoleoyl-[13C]glycerol and [12C]palmitoleoyl-[12C]glycerol, respectively. These results suggest that the palmitic acid was converted to palmitoleic acid in vivo by lipid-linked desaturation but not via a pathway consisting of deacylation, desaturation and reacylation.This publication has 1 reference indexed in Scilit:
- Glycerolipid synthesis in Avena leaves during greening of etiolated seedlings II. -Linolenic acid synthesisPlant and Cell Physiology, 1980