Improvement of Transduction Efficiency of Recombinant Adeno‐associated Virus Vector by Entrapment in Multilamellar Liposomes

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Abstract
Recombinant adeno‐associated virus (AAV) has attracted considerable interest as a potential vector for human gene therapy, but its transduction efficiency is quite low. The present study demonstrated AAV vector‐associated liposomes to be more effective for in vitro gene transfer to human glioma cells than are liposomes containing plasmid DNA. Using vector‐associated liposomes increased the transduction efficiency more than 10‐fold compared to liposomes containing plasmid DNA and more than 6‐fold compared to AAV alone. From these results, AAV vector‐associated liposomes appear to be a good candidate for in vivo gene delivery to human gliomas.