Flavonol 3-O-MethyItransferase in Plant Tissues
Open Access
- 1 February 1982
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 37 (1-2) , 134-135
- https://doi.org/10.1515/znc-1982-1-224
Abstract
Flavonol 3-O-methyltransferase activity has been detected in Calamondin orange peel and root tissue, tobacco cell culture and young shoots of Chrysosplenium americanum. The enzyme was purified from the latter tissue by precipitation with ammonium sulphate, ion-exchange chromatography and finally by chromatofocusing on Poly- buffer exchanger at pi 4.8. The focused enzyme exhibited strict stereospecificity towards quercetin, MW of 65000 daltons and pH optimum of 7.5-8.5. The apparent Km values for quercetin and S-adenosyl-ʟ-methionine were 1.4 and 70 μM, respectively.Keywords
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