Quantitative Kinetic Analysis in a Microfluidic Device Using Frequency-Domain Fluorescence Lifetime Imaging
- 2 May 2007
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 79 (11) , 4101-4109
- https://doi.org/10.1021/ac070045j
Abstract
A novel microfluidic approach for the quantification of reaction kinetics is presented. A three-dimensional finite difference numerical simulation was developed in order to extract quantitative kinetic information from fluorescence lifetime imaging experimental data. This approach was first utilized for the study of a fluorescence quenching reaction within a microchannel; the lifetime of a fluorophore was used to map the diffusion of a quencher across the microchannel. The approach was then applied to a more complex chemical reaction between a fluorescent amine and an acid chloride, via numerical simulation the bimolecular rate constant for this reaction was obtained.Keywords
This publication has 17 references indexed in Scilit:
- Microfluidic voltammetry: simulation of the chronoamperometric response of microband electrodes sited within microreactorsJournal of Solid State Electrochemistry, 2006
- The application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devicesOptics Express, 2006
- Quantitative Spatial Mapping of Mixing in Microfluidic SystemsAngewandte Chemie International Edition in English, 2005
- Measuring reaction kinetics in a lab-on-a-chip by microcoil NMRLab on a Chip, 2005
- A microchannel solution mixer for studying microsecond protein folding reactionsReview of Scientific Instruments, 2004
- Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopyBiochemical Journal, 2003
- Dynamic Fluorescence Anisotropy Imaging Microscopy inthe Frequency Domain (rFLIM)Biophysical Journal, 2002
- Formation of Natural pH Gradients in a Microfluidic Device under Flow Conditions: Model and Experimental ValidationAnalytical Chemistry, 2000
- Experimental and theoretical scaling laws for transverse diffusive broadening in two-phase laminar flows in microchannelsApplied Physics Letters, 2000
- Fluorescence lifetime imaging with picosecond resolution for biomedical applicationsOptics Letters, 1998