Partial Separation of Blood Clotting Factors, Albumin, and IgG by Continuous Free Film Electrophoresis
- 28 February 1983
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 44 (3) , 151-155
- https://doi.org/10.1111/j.1423-0410.1983.tb01877.x
Abstract
Blood plasma and factor VIII concentrate were fractionated by continuous free film electrophoresis in an apparatus which was designed to overcome the problems of overheating usually inherent in this technique. Complete separation of plasma proteins is unlikey to be obtained by a single passage through this apparatus. A useful degree of fractionation may be obtained with certain proteins, e.g., fibrinogen and IgG. It should be possible to concentrate blood clotting factors if the electrophoresis is used in combination with a conventional separation procedure such as adsorption on Al(OH)3. The advantage of the electrophoretic technique is that it is a continuous process using the minimum of extraneous chemicals. The disadvantage is that it introduces a 40-fold dilution into the fractionation.This publication has 1 reference indexed in Scilit:
- The use of thin layers in electrophoretic separationTransactions of the Faraday Society, 1940