Gene localization by chromosome fractionation: globin genes are on at least two chromosomes and three estrogen-inducible genes are on three chromosomes.

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Abstract
Chicken metaphase chromosomes were partially purified by rate zonal centrifugation, and DNA was prepared from each of the fractions of the sucrose gradient. The DNA was digested with various restriction enzymes and subjected to electrophoresis in agarose gels. The DNA was transferred to nitrocellulose filters (as described by Southern), and the filters were hybridized with c[complementary]DNA probes. Four globin genes .alpha.A, .alpha.D, .beta. and .rho. or .epsilon. are located on at least 2 chromosomes, and 3 of the estrogen-inducible genes of the hen oviduct ovalbumin, ovomucoid and transferrin, are on 3 different chromosomes. The earlier assignment of the endogenous viral sequence related to Rous-associated virus-O to a separate (and larger) chromosome than the cellular sequence related to the transforming gene of avian sarcoma virus (cellular sarc) is confirmed, although it now appears that cellular sarc is on a small macrochromosome, rather than on a microchromosome.