p-Maleimidophenyl isocyanate: a novel heterobifunctional linker for hydroxyl to thiol coupling
- 1 May 1993
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 4 (3) , 212-218
- https://doi.org/10.1021/bc00021a005
Abstract
P-Maleimidophenyl isocyanate (PMPI, 1) is a heterobifunctional cross-linking agent useful for thiol to hydroxyl coupling. Several maleimide-activated compounds were prepared and characterized and then shown to be reactive with thiol-containing proteins. Examples include activation of vitamin B12, digoxigenin, digitoxigenin, estradiol, progesterone, and some serine-containing peptides.Keywords
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