Biopalynological Study ofLapageria roseaandIris Unguicularis: Storage of Pollen

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Abstract
A biopalynological study of two plants, Lapageria rosea Ruiz & Pav. and Iris unguicularis Poir., was carried out within the framework of the creation of a pollen bank at the Palynology Laboratory, National Museum of Natural History, Paris. Descriptions of fresh pollen, using LM, SEM and TEM, made it possible to establish a reference system based on pollen morphology and also to verify the bicellular state at anther dehiscence; it is generally believed to be a favourable state for in vitro germination and pollen preservation. In vitro germination tests were also carried out on fresh pollen under different conditions. For stored pollen, comparisons are established for different methods of preservation (at ‐18°C or ‐34°C), different processes of freeze‐drying and vacuum‐drying and different storage conditions. The preserved pollen was periodically tested in vitro to control pollen viability and its germination capacity. A comparison between bicellular and tricellular pollen is presented and could be studied in future research.