Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood.
- 1 August 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (8) , 4914-4917
- https://doi.org/10.1073/pnas.77.8.4914
Abstract
A simple and rapid method for the determination of human T lymphocyte subclasses in buffy coat preparations or whole blood is described. This technique uses flow cytometry to distinguish lymphocytes from other leukocytes on the basis of their light-scattering properties. Lymnphocyte subclasses were enumerated by cellular immunofluorescence; the immunofluorescent signals were produced by monoclonal antibodies to surface differentiation antigens on T cells. Conventional techniques of enumerating T lymphocyte subclasses entail time-consuming (up to 2 days) density gradient and E rosette enrichement, and require at least 20 ml of blood. The method described here uses as little as 50 microliters of whole blood for each antibody tested and produces results within in 2 hr.This publication has 16 references indexed in Scilit:
- A monoclonal antibody reactive with human peripheral blood monocytes.The Journal of Immunology, 1980
- Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineageProceedings of the National Academy of Sciences, 1980
- Human antibody reacts with a B-cell subset in man to induce B-cell differentiationNature, 1980
- Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression.The Journal of Experimental Medicine, 1979
- Abnormalities of Immunoregulatory T Cells in Disorders of Immune FunctionNew England Journal of Medicine, 1979
- Monoclonal Antibodies Defining Distinctive Human T Cell Surface AntigensScience, 1979
- A Monoclonal Antibody with Selective Reactivity with Functionally Mature Human Thymocytes and All Peripheral Human T CellsThe Journal of Immunology, 1979
- Separation of functional subsets of human T cells by a monoclonal antibody.Proceedings of the National Academy of Sciences, 1979
- Identification of five human lymphocyte subpopulations by their differential binding of various strains of bacteriaCellular Immunology, 1977
- Functional Analysis of Distinct Human T-Cell Subsets Bearing Unique Differentiation AntigensPublished by Springer Nature ,1977