Enzyme Activities During Culturing of Fetal Rat Liver

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Abstract
Liver from fetal rats was cultured in a simple medium. In such cultures tyrosine transaminase (TTA) activity had increased after 20 min and reached twice the initial value within 2 h. Phosphoenolpyruvate earboxykinase (PEPCK) activity decreased during culturing. Incubation of microsomes from fresh fetal liver with dibutyryl cyclic AMP (DcAMP), oleic acid, or acetyl-CoA led to an increase in their TTA activity. It is suggested that the early rise in TTA during culturing is due to release of the enzyme from the microsomal fraction. In contrast to human fetal liver, oleic acid did not induce PEPCK in rat fetal liver cultures. In neither species was there an effect of DcAMP on the amount of fatty acids in the culture medium or on the activities of carnitine acetyl- and carnitine palmitoyltransferases.

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