Evaluation of the leucocyte migration test as a measure of delayed hypersensitivty in man. Suppression of migration inhibition by puromycin.

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  • 1 August 1972
    • journal article
    • Vol. 11  (4) , 535-41
Abstract
The response of peripheral blood leucocytes to PPD was studied in vitro by means of the leucocyte migration test, using Mantoux-positive and -negative subjects. The migration of leucocytes from 83% of the positive subjects was significantly inhibited when a concentration of 100 μg PPD/ml was used in the chambers. Leucocytes from the majority of these subjects were also inhibited at a lower concentration of 50 μg PPD/ml and one strongly positive subject showed inhibition at all concentrations tested, down to 1 μg PPD/ml. Stimulation of migration was not observed with leucocytes from any of the positive subjects even when the lowest concentration of PPD was used. Inhibition of migration with PPD was prevented by the addition of puromycin, suggesting that the response is dependent on protein synthesis and may be mediated by a soluble factor produced by sensitized lymphocytes.