Titration of the binding sites for the oligomycin-sensitivity conferring protein in beef heart submitochondrial particles
- 30 May 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 156 (1) , 99-102
- https://doi.org/10.1016/0014-5793(83)80256-7
Abstract
The binding parameters of the oligomycin-sensitivity conferring protein (OSCP) in inside-out particles from beef heart mitochondria have been tested by means of two assays, the oligomycin-sensitive ATP—Pi exchange, and the oligomycin-sensitive ATP hydrolysis. The total number of OSCP binding sites in A particles was equal to 220 pmol/mg particle protein. Each mole of ATPase active site was able to bind 1.1±0.5 mol OSCP with K d 1.7 nM.Keywords
This publication has 16 references indexed in Scilit:
- Structure of oxidative- and photo-phosphorylation coupling factor complexesBiochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics, 1979
- Association of a thioredoxin-like protein with chloroplast coupling factor (CF1)Biochemical and Biophysical Research Communications, 1979
- [49] Oligomycin-sensitivity-conferring proteinPublished by Elsevier ,1979
- Proteins required for the binding of mitochondrial ATPase to the mitochondrial inner membraneBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- F1·X, a complex between F1 and OSCPBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
- On the relationship between the oligomycin-sensitivity conferring protein and other mitochondrial coupling factorsJournal of Bioenergetics and Biomembranes, 1971
- Mitochondrial adenosine triphosphatase system. IV. Purification and characterization of the oligomycin sensitivity-conferring proteinBiochemistry, 1968
- Mitochondrial adenosinetriphosphatase system. III. Isolation from the oligomycin-sensitive adenosinetriphosphatase complex of the factors which bind F1 and determine oligomycin sensitivity of bound F1Biochemistry, 1968
- [9] Measurement of ATPase, 14C-ADP-ATP, and 32Pi-ATP exchange reactionsPublished by Elsevier ,1967