A novel method for evaluating the outer membrane permeability to .BETA.-lactamase-stable .BETA.-lactam antibiotics.
- 1 January 1980
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 33 (3) , 310-316
- https://doi.org/10.7164/antibiotics.33.310
Abstract
A novel method is described which allows estimation of the outer membrane permeability to .beta.-lactamase-stable .beta.-lactams by determining antibiotic concentrations in the periplasm. The method is based on measurement of the inhibiting activity of .beta.-lactamase-stable .beta.-lactams on the hydrolysis of a substrate by periplasmic .beta.-lactamase. Application of the method to carbenicillin revealed that the high level of resistance to carbenicillin of an Escherichia coli strain acquiring the plasmid encoding ampicillin resistance resulted from the poor ability of carbenicillin to penetrate the outer membrane of E. coli.This publication has 3 references indexed in Scilit:
- The bacterial outer-membrane permeability of .BETA.-lactam antibiotics.The Journal of Antibiotics, 1979
- Identification of the outer membrane protein of E.coli that produces transmembrane channels in reconstituted vesicle membranesBiochemical and Biophysical Research Communications, 1976
- Outer membrane of Salmonella typhimurium: accessibility of phospholipid head groups to phospholipase C and cyanogen bromide activated dextran in the external mediumBiochemistry, 1976