Cryopreservation of zebra fish spermatozoa using methanol
- 1 August 1982
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Zoology
- Vol. 60 (8) , 1867-1870
- https://doi.org/10.1139/z82-242
Abstract
We describe a method for cryopreservation of milt from individual Brachydanio rerio using methanol and powdered milk as cryoprotectants. Motility was positively correlated with hatching, which averaged 51 ± 35.6% in a typical experiment. Variablity in motility and hatching was not correlated with sperm volume or age of the fish, and is believed to be due to differences in sperm quality between individuals, as well as technical constraints imposed by the short duration of motility in thawed spermatozoa.This publication has 2 references indexed in Scilit:
- Cryoprotectant penetration and supercooling in the eggs of salmonid fishesCryobiology, 1982
- Water permeability in the developing embryo of the zebrafish, Brachydanio rerioCanadian Journal of Zoology, 1982