Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI-ion mobility-TOFMS
- 1 February 2005
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Society for Mass Spectrometry
- Vol. 16 (2) , 133-138
- https://doi.org/10.1016/j.jasms.2004.10.002
Abstract
After water, lipids are the most common biomolecules found in the brain (12%). A brief perusal of the physiology, anatomy, and pathophysiology of the brain illustrates the importance of lipids. Recent advances in mass spectrometry have allowed the direct probing of tissues. However, most studies have focused on proteins. In the present work, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and MALDI-ion mobility (IM)-TOFMS were employed for direct analysis of phospholipids in rat brain tissue. Molecular ions (MH+) corresponding to phosphatidylcholines, phosphatidylethanolamines, and sphingomyelin, were recorded. When studying pharmacology, we learn that many therapeutic compounds are stored in the body’s adipose tissue. MALDI-TOFMS and MALDI- IM-TOFMS were thus used to analyze rat brain tissue with chlorisondamine added directly onto the tissue slice. With both techniques, noncovalent complexes between the tissue phospholipids and chlorisondamine were detected. In addition, MALDI-IM-TOFMS of noncovalent complexes between phospholipids and chlorisondamine displayed a mobility between that of an isobaric lipid and peptide.Keywords
This publication has 25 references indexed in Scilit:
- Lipid/Peptide/Nucleotide Separation with MALDI-Ion Mobility-TOF MSAnalytical Chemistry, 2004
- The Mighty Arginine, the Stable Quaternary Amines, the Powerful Aromatics, and the Aggressive Phosphate: Their Role in the Noncovalent MinuetJournal of Proteome Research, 2004
- Direct tissue analysis using matrix‐assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparationJournal of Mass Spectrometry, 2003
- Interaction of Chlorisondamine with the Neuronal Nicotinic Acetylcholine ReceptorJournal of Proteome Research, 2003
- Direct profiling and imaging of peptides and proteins from mammalian cells and tissue sections by mass spectrometryElectrophoresis, 2002
- Substantial sulfatide deficiency and ceramide elevation in very early Alzheimer's disease: potential role in disease pathogenesisJournal of Neurochemistry, 2002
- A Fluorescence-Based, High-Throughput Sphingomyelin Assay for the Analysis of Niemann–Pick Disease and Other Disorders of Sphingomyelin MetabolismAnalytical Biochemistry, 2002
- Phospholipid composition and levels are altered in down syndrome brainBrain Research, 2000
- Application of delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry for analysis of sphingolipids in tissues from sphingolipidosis patientsJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- THE CHEMICAL COMPOSITION OF VERTEBRATE MYELIN AND MICROSOMESJournal of Neurochemistry, 1965