New structures in complex formation between DNA and cationic liposomes visualized by freeze—fracture electron microscopy
Open Access
- 19 December 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 356 (2-3) , 361-366
- https://doi.org/10.1016/0014-5793(94)01315-2
Abstract
Structures formed during interaction of cationic liposomes and plasmid DNA were studied by freeze—fracture electron microscopy and their morphology was found to be dependent on incubation time and DNA concentration. These structures were formed with liposomes composed of DC‐Chol and DOPE after 30 min incubation at DNA: lipid concentrations encompassing maximal transfection activity. They resembled liposome complexes (meatballs) and additionally bilayer‐covered DNA tubules (spaghetti), whereby the DNA‐tubules were found to be connected to the liposome complexes as well as occurring free in the suspension. At later times and higher DNA‐to‐liposome ratios the complexes grow larger while their membranes become discontinuous, allowing the self‐encapsulation of the DNA. The relative transfection potency of the various morphologically distinct structures is discussed.Keywords
This publication has 28 references indexed in Scilit:
- Lipid polymorphism and the functional roles of lipids in biological membranesPublished by Elsevier ,2002
- The Twist, Writhe and Overall Shape of Supercoiled DNA Change During Counterion-induced Transition from a Loosely to a Tightly Interwound SuperhelixJournal of Molecular Biology, 1994
- A novel cationic liposome reagent for efficient transfection of mammalian cellsBiochemical and Biophysical Research Communications, 1991
- Liposomes for the transformation of eukaryotic cellsBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1991
- Supramolecular organization of double-stranded DNA molecules in the columnar hexagonal liquid crystalline phaseJournal of Molecular Biology, 1991
- Differentiation expression during proliferative activity induced through different pathways: in situ hybridization study of thyroglobulin gene expression in thyroid epithelial cells.The Journal of cell biology, 1990
- Structure of the inverted hexagonal (HII) phase, and non-lamellar phase transitions of lipidsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1990
- Use of a quaternary ammonium detergent in liposome mediated DNA transfection of mouse L-cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1989
- Rapid Communication: In vivo Transfection of Murine Lungs with a Functioning Prokaryotic Gene using a Liposome VehicleThe Lancet Healthy Longevity, 1989
- THE ADHESIVENESS OF LEUKOCYTES WITH SPECIAL REFERENCE TO ZETA POTENTIALAnnals of the New York Academy of Sciences, 1964