Effeets of hyperthermia at 50.DEG.C on V-79 cells in vitro.
Open Access
- 1 January 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Radiation Research
- Vol. 23 (2) , 218-227
- https://doi.org/10.1269/jrr.23.218
Abstract
V-79 cells were X-irradiated and/or heated at 42°C, 44°C and 50°C in water bath and colonized in vitro. To and Tq at 50°C were estimated as about 0.1 minute and theoretically less than 1.0 minute respectively. Arrhenius like plot did'nt bend at any temperatures between 43°C and 50°C, as reported elsewhere1). Preheated cells at 42°C for 2 hours were resistant to the successive heating at 50°C, while those at 50°C for 80 seconds were sensitized to that at 42°C. The survival fraction ratio, i.e. products of survival fractions from independent treatment with 4.5 Gy and with 50°C, respectively, divided by a survival fraction from a combined treatment with 4.5 Gy and 50°C, showed that the longer 50°C heating period gave the more synergistic damage. Postirradiation heating periods were conditioned to give the equal survivals from single heat treatment at 42°C, 44°C and 50°C for the estimation of thermal enhancement ratio in Do, i.e. 1.98, 1.98 and 1.64 respectively. Heating at 50°C showed the given cell killing rate for the shortest period among the temperatures assayed but did'nt seem essentially to give the more thermal radio-enhancement ratio by itself.This publication has 1 reference indexed in Scilit: