Application of polystyrene‐bound invertase to continuous sucrose hydrolysis on pilot scale

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Abstract
Invertase from baker's yeast (Saccharomyces cerevisiae) covalently bound to a macroporous polystyrene anion‐exchange resin via glutaraldehyde was applied to continuous sucrose hydrolysis in packed bed‐reactors. The process was scaled up from 3‐mL laboratory reactors via 0.3‐L reactors to pilot‐scale 50‐L reactors without significant loss of efficiency. The described process allows the production of a wide spectrum of invert sugar syrups with high purity in continuous procedure. The 50‐L reactor was used under process conditions 1 year without significant loss of productivity at a temperature of 40°C. A productivity of 760 g/h was obtained with 1 L invertase–polystyrene complex using a 2.5M sucrose solution as substrate. © 1992 John Wiley & Sons, Inc.