CALCIUM-UPTAKE BY ISOLATED SARCOPLASMIC-RETICULUM - EXAMINATION OF HALOTHANE INHIBITION, PH-DEPENDENCE, AND CA2+ DEPENDENCE OF NORMAL AND MALIGNANT HYPERTHERMIC HUMAN-MUSCLE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 60  (7) , 492-498
Abstract
Ca2+ uptake and release in muscle homogenates and fragmented sarcoplasmic reticulum were examined in biopsy specimens from nonsusceptible and malignant hyperthermia (MH) susceptible patients. Ca2+ flux was examined by the filter binding assay technique using 45Ca. Ca2+ uptake and release were the same in both normal and MH muscle homogenates. Halothane inhibited the uptake of Ca2+ by the sarcoplasmic reticulum. The halothane inhibition of Ca2+ uptake in normal and MH sarcoplasmic reticulum was fitted to a single line with a correlation coefficient (r) of -0.958. The pH and Ca2+ dependence of Ca2+ uptake were the same for both normal and MH sarcoplasmic reticulum. The pK for Ca2+ uptake is .apprx. 5.9. Ca2+ uptake function of the muscle from the 5 patients with MH examined is not abnormal and might not be the locus for the initiation of MH.

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