Temperature‐dependent conformational changes in isolated oligomycin‐sensitive ATPase
- 2 May 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 155 (1) , 131-134
- https://doi.org/10.1016/0014-5793(83)80224-5
Abstract
Isolated oligomycin-sensitive ATPase undergoes a kinetic change at 20–25°C with a higher activation energy and a lower K m for ATP below this temperature range. This observation has been correlated with temperature-dependent structural changes detected by circular dichroism in the UV region in the isolated enzyme. The negative ellipticities in the 208-225 nm region, which are proportional to the α-helix content, increase with rise in temperature to a maximum above 25°C.Keywords
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