Ultrafiltration and Electron Microscopy of Three Types of Poliomyelitis Virus Propagated in Tissue Culture.

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Abstract
When the concn. of virus in stock filtrates of the tissue culture fluids was greater than 106TCD50 per ml., membranes with an APD of 39 m[mu]-44m[mu] allowed a certain proportion of poliomyelitis virus particles to pass. Membranes with an APD of 36-39 m[mu] held back the virus and, according to Elford''s formula, the size of all 3 types of poliomyelitis virus is estimated at 12-20 m[mu]. After removal of gross amorphous material by preliminary centrifugation, the tissue culture fluids yielded practically pure suspensions of virus particles which measured, on the avg., 30 m[mu] for Type 1 (Mahoney), 27 m[mu] for Type 2(Y-SK), and 41 m[mu] for Type 3 (Leon). The seemingly larger size of the Type 3 particles may be an artifact.

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