Recombinant humanO6-alkylguanine-DNA alkyltransferase induces conformational change in bound DNA
Open Access
- 5 May 1997
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 407 (3) , 333-336
- https://doi.org/10.1016/s0014-5793(97)00370-0
Abstract
Circular dichroism, and steady‐state and time‐resolved fluorescence spectroscopy were used to compare the native recombinant human DNA repair protein O 6‐alkylguanine‐DNA alkyltransferase (AGT) with AGT bound to ds‐DNA. Contrary to fluorescence, analysis of the far‐UV CD spectra indicated a conformational change of AGT upon binding to DNA: its α‐helical content is increased by ∼12%. Analysis of near‐UV CD spectra revealed that DNA was also affected, probably being separated into single strands locally.Keywords
This publication has 21 references indexed in Scilit:
- Structure, Function, and Inhibition of O6-Alkylguanine-DNA AlkyltransferasePublished by Elsevier ,2008
- Binding and repair of O6-ethylguanine in double-stranded oligodeoxynucleotides by recombinant human O6-alkylguanine-DNA alkyltransferase do not exhibit significant dependence on sequence contextNucleic Acids Research, 1996
- Enzyme Structures: DNA repair flips outCurrent Biology, 1995
- Tryptophan mutants of human C5a anaphylatoxin: A fluorescence anisotropy decay and energy transfer studyBiophysical Chemistry, 1993
- Kinetic and DNA-Binding Properties of Recombinant Human O6-Methylguanine-DNA MethyltransferaseArchives of Biochemistry and Biophysics, 1993
- Protein secondary structure and circular dichroism: A practical guideProteins-Structure Function and Bioinformatics, 1990
- A solution equivalent of the 2Zn→4Zn transformation of insulin in the crystalEuropean Journal of Biochemistry, 1984
- Estimation of globular protein secondary structure from circular dichroismBiochemistry, 1981
- Two-Point Calibration of Circular Dichrometer with d-10-Camphorsulfonic AcidAnalytical Letters, 1977
- Different conformations of double‐stranded nucleic acid in solution as revealed by circular dichroismBiopolymers, 1973